Investigation of the chemical composition and different effects of a rumex dentatus methanol extract against drug resistant pseudomonas aeruginosa isolates

Background: The emergence of Pseudomonas aeruginosa as a multiple drug resistant bacteria has renewed efforts to identify alternative antibacterial materials,such as medicinal plants.Objectives: The aims of this study were to investigate the antibacterial effects of a Rumex dentatus methanol extract against drug resistant P.aeruginosa isolates and to analyze the chemical composition of this extract.Materials and Methods: Sixty strains of P.aeruginosa were isolated from urine and wound samples and identified using conventional tests,as well as 16S rDNA gene sequencing.Results: The isolates were completely resistant to the tested antibiotics,with minimum inhibitory concentration (MIC) values for ceftazidime,imipenem,and gentamicin between 256 to >256 µg/mL,8 to >256 µg/mL,and 64 to >256 µg/mL,respectively.The R.dentatus extract had a broad spectrum activity against all tested isolates and maximum growth inhibition against isolates from burn wound and urinary tract infections occurred at 200 mg/mL (22.43mm and 22.86mm,respectively).The means of the MIC and minimum bactericidal concentration (MBC) values were 25 and 50 mg/mL,respectively.The effects of a combination of the methanol extract and ceftazidime,imipenem,and gentamicin antibiotics were antagonistic.The GC-MS analysis of the methanol extract revealed a high probable presence percentage of alpha-pinene,sabinene,and eucalyptol (1,8-cineole) that have confirmed antimicrobial activity.Conclusions: The R.dentatus methanol extract with broad spectrum activity can be viewed as an alternative to antibiotics for treatment of P.aeruginosa infection.© 2016,Iranian Red Crescent Medical Journal.

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