Xenotropic murine leukemia virus-related virus and RNase L R462Q variants in Iranian patients with sporadic prostate cancer

Background: Although several studies have confirmed the association of xenotropic murine leukemia virus-related virus (XMRV) and prostate cancer,this association is still controversial,as most studies did not detect XMRV in prostate tissue samples.Furthermore,some genetic and epidemiological studies have highlighted a role for RNase L polymorphisms,particularly R462Q,in the progression of prostate cancer.Objectives: The focus of this study was on the association of XMRV and RNase L R462Q variants with the risk of prostate cancer in Iranian patients.Patients and Methods: In this case-control study,40 and 80 individuals with sporadic prostate cancer and benign prostatic hyperplasia,respectively,were included.The presence of XMRV was evaluated by real-time polymerase chain reaction (PCR) of integrase and nested-PCR for the gag genes.The RNase L R462Q polymorphism analysis was carried out by PCR and sequencing.Results: In a total of 40 sporadic prostate cancer and 80 benign prostatic hyperplasia cases,no XMRV was detected by real-time PCR and nested-PCR.RNase L R462Q polymorphism analysis reveals that although there was an increase in the risk of prostate cancer correlated with the Q/Q allele of RNase L at position 462,the frequencies of the RNase L R462Q alleles were not statistically significant between the prostate cancer and benign prostatic hyperplasia groups (OR = 2.75 (95% CI = 0.67-11.3),P = 0.29).Conclusions: These results did not support the presence of XMRV in the samples with prostate cancer and showed that RNase L R462Q variants had relatively little or no impact on the risk of prostate cancer in Iranian population.© 2015,Iranian Red Crescent Medical Journal.

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